Composite
Part:BBa_K3453160:Design
Designed by: Maeva Cherriere Group: iGEM20_Evry_Paris-Saclay (2020-10-04)
Rosewood DmTrnL-UAA Trigger (Full) transcription unit
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 381
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
T7 promoter (BBa_K2150031) + Rosewood DmTrnL-UAA Trigger (Full) (BBa_K3453060) + the strong SBa_000587 Terminator (BBa_K3453000)
Source
DNA synthesis & Assembly
References
[1] Green AA, Silver PA, Collins JJ, Yin P. Toehold switches: de-novo-designed regulators of gene expression. Cell (2014) 159, 925-939.